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Siemens AG
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Fisher Scientific
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Matsunami Glass
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Microm International GmbH
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Image Search Results
Journal: Wellcome Open Research
Article Title: ultraLM and miniLM: Locator tools for smart tracking of fluorescent cells in correlative light and electron microscopy
doi: 10.12688/wellcomeopenres.10299.1
Figure Lengend Snippet: ( A ) Schematic of the interior of the 3View SBF SEM chamber showing relative positions of major components and the location of the 3 mm gap between the top of the sample and the bottom of the BSE detector. ( B ) Schematic of the optical arrangement of the miniLM. ( C ) Schematic of the custom lens in the miniLM alongside a photograph showing the physical dimensions. The miniature microscope objective is attached to the end of a coherent fibre bundle, which serves to feed the image out of the vacuum chamber. ( D ) Schematic of the coherent fibre bundle. An image presented to the proximal end of the fibre is relayed in a spatially coherent manner to the distal end. Note the resultant pixellation of the image due to the finite number of fibre cores. ( E ) Transmission image of a USAF target to demonstrate the resolution of the miniLM. The smallest lines on the target are 2.18 µm wide with equal separation distance, which we were able to resolve. The inset shows a line profile taken through the lines of Group 7, Element 6 of the USAF resolution target, confirming a resolution of better than 2.18 µm. The smaller background peaks were caused by the pixellation due to the discrete fibre cores. The target was illuminated with a white light LED through an objective of similar NA to the miniature objective.
Article Snippet: The delay time until the motor was restarted at the miniLM imaging position was set to 1.5 s. The serial imaging run was set up and started using the
Techniques: Microscopy, Transmission Assay